| 朱炜,杨可武,姜玄珍.化学酶法合成光学纯亮氨酸[J].分子催化,2005,(5):399-402 |
| 化学酶法合成光学纯亮氨酸 |
| Chemoenzymatic Synthesis of Enantiomerically Pure Leucine |
| 投稿时间：2005-03-07 修订日期：2005-04-27 |
| DOI： |
| 中文关键词: 化学酶法合成 酰胺羰基化 光学纯亮氨酸 酶水解 |
| 英文关键词:Chemoenzymatic synthesis Amidocarbonylation Enantiomerically pure leucine Enzymatic hydrolysis |
| 基金项目:中国科学院资助项目 |
| 朱炜 杨可武 姜玄珍 |
| 浙江大学玉泉校区化学系,浙江杭州310027 |
| 摘要点击次数: 1187 |
| 全文下载次数: 14 |
| 中文摘要: |
| 用化学酶法合成L-亮氨酸，先在PdCl2/PPh3/LiBr/H2SO4催化体系作用下，异戊醛酰胺羰基化合成消旋的N-乙酰基亮氨酸；然后在酰基转移酶催化下水解，得到L-亮氨酸和N-乙酰基-D-亮氨酸．文中对酰胺羰基化反应条件进行了优化，分离得到消旋N-乙酰基亮氨酸产率为66．4％．经酶对映选择地水解后得L-亮氨酸，产率为41％，产物L-亮氨酸经衍生化后由气相色谱测定其光学纯度达99％ee． |
| 英文摘要: |
| In this study enantiomerically L-leucine was prepared by chemoenzymatic synthesis.In the first step of the reaction sequence the racemic N-acetylleucine was prepared by amidocarbonylation from isovaleraldehyde,acetamide and carbon monoxide in the presence of PdCl_2/PPh_3/LiBr/H_2SO_4 catalyst system.Subsequently L-leucine andD-N-acetylleucine were obtained by the acylase-catalyzed hydrolysis.The amidocarbonylation reaction conditions were optimized and 66.4% isolated yield of racemic N-acetylleucine were obtained.L-leucine was obtained from the enzymatic enantioselective hydrolysis of racemic N-acetylleucine in a yield of 41%,and its derivative enantiomeric purity determined by G.C was 99% ee. |
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