| 辛嘉英.无溶剂体系脂肪酶催化合成阿魏酸油醇酯[J].分子催化,2011,(3):262-268 |
| 无溶剂体系脂肪酶催化合成阿魏酸油醇酯 |
| Lipase-Catalyzed Synthesis of Oleyl Ferulate in Solvent-Free System |
| 投稿时间：2011-04-28 修订日期：2011-06-15 |
| DOI： |
| 中文关键词: 阿魏酸乙酯 油醇 阿魏酸油醇酯 Novozym435脂肪酶 无溶剂体系 |
| 英文关键词:ethyl ferulate fatty alcohol fatty ferulate Novozym435 lipase solvent-free system |
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| 摘要点击次数: 1429 |
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| 中文摘要: |
| 文对无溶剂体系中阿魏酸的转酯化疏水改性进行了研究,确立了减压反应器（0.001 MPa）中Novozym 435脂肪酶催化阿魏酸乙酯和油醇进行转酯化反应合成新型抗氧化剂阿魏酸油醇酯的方法。发现水活度(aw) 明显影响转酯反应,阿魏酸油醇酯产率在aw < 0.01-0.75 范围内随着水活度的增加而降低。推测底物阿魏酸乙酯和产物阿魏酸油醇酯的水解是该转酯反应的竞争性抑制因素。同时以阿魏酸油醇酯的产率为考察指标，探讨了温度、加酶量、搅拌器转速、反应时间、水活度、底物摩尔比等因素对转酯化反应的影响。确定了脂肪酶催化阿魏酸油醇酯合成的最佳反应条件为：5.0 mmol油醇，1.0 mmol阿魏酸乙酯，70 mg Novozym435脂肪酶，水活度小于0.01，反应温度为60℃，转速为200 r/min,反应时间为96 h，此时阿魏酸油醇酯的产率可达65%。固定化酶反复使用多次后，活性没有明显损失。 |
| 英文摘要: |
| Lipase-catalyzed synthesis of a new-type of antioxidant in solvent-free system was studied. Using Novozym435 as catalyst, transesterification of ethyl ferulate (EF) with different fatty alcohol to form fatty ferulate in solvent-free system was investigated. According to the yield of transesterification, ethyl ferulate (EF) with oleyl alcohol (OA) was chosen as the optimized substrate. The influences of reaction temperature, the ratio of enzyme to substrate, agitation speed, reaction time, water activity, the molar ratio of substrates, vacuum-rotary evaporation procedure and the stability of enzyme on the yield of oleyl ferulate were studied in detail.The process was optimized for the enzymatic synthesis of oleyl ferylate using a vacuum-rotary evaporation procedure under following conditions: 5.0 mmol oleyl alcohol, 1.0 mmol ethyl ferulate, 70 mg Novozym 435, water activity(aw)﹤0.01, at 60 ℃, 200 r/min agitation and 0.001 MPa for 96 h. The optimized conditions applied to the transesterification of ethyl ferulate with oleyl alcohol obtain oleyl ferulate yield of 65%. The enzyme can be reused several times without significant loss of activity. |
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