| 王梦亮,姜骉麟,崔晋龙,焦红卫.表面活性剂在水-有机两相体系中对β-葡萄糖苷酶催化合成红景天苷的影响[J].分子催化,2013,(1):83-88 |
| 表面活性剂在水-有机两相体系中对β-葡萄糖苷酶催化合成红景天苷的影响 |
| Effect of Surfactants on the β-Glycosidase Enzymatic Synthesis of Salidroside in Aqueous-organic Biphasic Systems |
| 投稿时间：2012-11-30 修订日期：2013-01-01 |
| DOI： |
| 中文关键词: 生物催化 β-葡萄糖苷酶 逆水解反应 表面活性剂 |
| 英文关键词:bio-catalysis β-glycosidase reverse hydrolysis surfactant |
| 基金项目: |
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| 摘要点击次数: 1667 |
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| 中文摘要: |
| 采用缓冲液/正己烷为反应体系，比较了几类表面活性剂(CTAB，SDS，SDBS，Tween 20)对酶活的影响。并在该反应体系中，考察了表面活性剂对β-葡萄糖苷酶催化合成红景天苷的反应中底物转化率以及初始反应速率的影响，确定了反应混合体系的适宜含水量。结果表明，在水/有机两相体系中，HLB值较高的SDS对酶的失活体现出了一定的抑制作用，其余表面活性剂均对酶失活起了不同程度的加速作用。在几类表面活性剂各自最适添加浓度下，CTAB，SDS， Tween 20三组均将底物转化率从9.2%提高到11%左右。不添加表面活性剂所测得的初始反应速率最高，Tween 20组次之，离子型表面活性剂添加组最低。对于SDS添加组，当其含水量为10%时，底物转化率可以达到22.3%。 |
| 英文摘要: |
| β-Glycosidase form almond seed was used as catalyst for the synthesis of salidroside. Enzyme activity in the presence of surfactants (CTAB, SDS, SDBS and Tween 20) was studied by assaying for enzyme activity in aqueous-organic biphase. SDS was found to be the most effective at maintaining enzyme activity in the aqueous-organic biphasic systems. The effect of surfactant on the substrate conversion in the aqueous-n-hexane biphasic system was investigated, too. The conversion of glucose was enhanced to 11.6% by adding 0.75 g/L SDS in the reaction system. Glucose conversion of 11% was achieved by adding CTAB of 1.25 g/L and Tween 20 of 1.75 g/L, respectively. For the SDS treatment, a substrate conversion rate of 22.3% was obtained under the condition of water content of 10% in the biphasic systems. |
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