王艳,辛嘉英,石佳.微波辅助酶促月桂酸淀粉酯的合成[J].分子催化,2014,(1):67-74
微波辅助酶促月桂酸淀粉酯的合成
Lipase-Catalyzed Esterification of Starch Using Lauric acid Under Microwave
投稿时间:2013-12-09  修订日期:2014-01-04
DOI:
中文关键词:  淀粉预处理  微波辐照  脂肪酶  月桂酸淀粉酯
英文关键词:starch pretreatment, microwave irradiation, lipase, lauric acid starch ester
基金项目:国家自然科学基金(21073050),黑龙江省杰出青年基金( jc201106) ,黑龙江省教育厅科技创新团队计划( 2010td04 ) ,教育厅重点项目 ( 11551z007)
作者单位E-mail
王艳* 哈尔滨商业大学 wangyan_123456@163.com 
辛嘉英 哈尔滨商业大学 xinjiayingvip@163.com 
石佳 哈尔滨商业大学 shijiaajia@126.com 
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中文摘要:
      以天然玉米淀粉和月桂酸为原料,脂肪酶Novozym 435为催化剂,研究了微波辐照下月桂酸淀粉酯的酶促合成。通过对淀粉进行预处理活化来提高淀粉的酯化反应活性,并主要考察了微波功率、月桂酸用量、脂肪酶Novozym 435用量及反应时间等参数对酶促月桂酸淀粉酯合成的影响。采用气相色谱法进行取代度的测定,并以取代度为考察指标,确定了最佳的反应条件。结果表明,淀粉的最佳预处理方法为NaOH/尿素混合溶液法,经该法处理后淀粉的酯化反应活性大大提高;微波辐照技术的应用大大缩短了反应时间,并提高了月桂酸淀粉酯的取代度。适宜的工艺条件为:微波功率为240W,月桂酸用量为30%,酶加入量为7%,反应时间26min。在上述反应条件下可以制得取代度为 0.0311的月桂酸玉米淀粉酯。
英文摘要:
      Lauric acid starch ester was synthesized by using immobilized lipase Novozym435 as the biocatalyst under microwave. The esterification activity was been improved by pretreatment. Several factors, such as the microwave power, amount of lipase Novozym 435, amount of lauric acid and esterification time has been investigated. A novel GC -based method was used to measure the degree of substitution (DS) of lauric acid starch ester. DS as the research object was adopt to optimize the reaction conditions. According to the results of the single-factor experiments, the perpendicular experiments were designed to optimize the parameters of the reaction. The results showed that the optimum reaction conditions were as below: microwave power was 240W, the amount of lauric acid was 30%, the amount of lipase was 7% and the reaction time was 26min. A degree of substitution of 0.0311 was obtained. The structures of products were characterized by FT-IR spectra.
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