| 辛嘉英,黄友梅.以改性Al2O3为载体固定化葡萄糖氧化酶的研究[J].分子催化,1993,(6):446-452 |
| 以改性Al2O3为载体固定化葡萄糖氧化酶的研究 |
| Study on the Treated A1_2O_3 Carrier Immobilization of GOD |
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| DOI： |
| 中文关键词: 葡萄糖氧化酶 过氧化氢酶 固定化 |
| 英文关键词:Glucose oxidase, Catalase, Mn2O3 , Immobilization, Crosslinking. |
| 基金项目:国家自然科学基金资助项目 |
| 辛嘉英 黄友梅 |
中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室 兰州730000
(辛嘉英)
，中国科学院兰州化学物理研究所羰基合成与选择氧化国家重点实验室 兰州730000(黄友梅)
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| 中文摘要: |
| 本文采用吸附和交联、生物和非生物催化剂结合的方法,制得活性高、稳定性好并以Al_2O_3.为载体的固定化葡萄糖氧化酶,其性能优于文献报导的结果. |
| 英文摘要: |
| In this study glucose oxidase (GOD) can be adsorbed on Al2O3 inorganic carrier followed by crosslinking with glutaraldehyde. In some cases superior results are obtained when the support is pretreated with TiCl4. The GOD catalysts co-immobilized with a very effective inorganic peroxide decomposition catalyst (Mn2O3) or an organic peroxide decomposition catalyst catalase (CAT) have higher activity and stability. Immobilized GOD made by different methods give superior results. The activities of two immobilized GOD are 470 u/g and 487 u/g, and the half-life 1216. 0 h and 1415. 9 h respectively |
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