| 刘文芳.碳酸酐酶固定化研究进展[J].分子催化,2016,30(2):182-197 |
| 碳酸酐酶固定化研究进展 |
| Research progress on carbonic anhydrase immobilization |
| 投稿时间：2016-02-03 修订日期：2016-02-29 |
| DOI： |
| 中文关键词: 碳酸酐酶 固定化 载体 酶活 稳定性 |
| 英文关键词:carbonic anhydrase immobilization carrier enzyme activity stability |
| 基金项目:国家自然科学基金项目（面上项目，重点项目，重大项目） |
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| 中文摘要: |
| 近年来，碳酸酐酶（CA）在CO2捕集领域的应用引起人们极大的兴趣。然而，由于价格昂贵，游离酶在使用时活性易受到多种环境因素的影响，稳定性较低且不易回收，因此有必要对CA固定化。综述了近十多年来CA固定化的研究进展，并根据载体的种类进行划分，分别总结了高分子材料、无机材料、聚合物-无机复合材料、纳米材料固定化CA的酶来源、固定化方法、载体材料、酶活、反应动力学参数和稳定性数据，并介绍了固定化CA应用于反应器的研究进展。最后，指出了不同类型载体材料的优缺点，提出了固定化CA的未来发展方向。 |
| 英文摘要: |
| In recent years, the application of carbonic anhydrase (CA) in CO2 capture has attracted great interest. However, as most other enzymes, on one hand, free CA has poor stability and the activity is susceptible to a variety of environmental factors, on the other hand, it is expensive and not easy to recycle. Therefore, it is necessary to immobilize CA for economic reason. This paper summarized the research progress on CA immobilization in the past decade. The type of the carrier was classified into four types: polymer, inorganic matrix, polymer-inorganic composite, nanometer materials, and the source of enzyme, immobilization method, support material, enzyme activity, reaction kinetic parameters and the stability of enzyme immobilized on each type of carrier have been summarized and listed. Research progress on the application of immobilized CA in reactor has been reviewed. At last, the advantages and disadvantages of all kinds of supporting materials were pointed out and the existing problems and future research direction of CA immobilization were presented. |
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