| 卢佳伟,李由然,石贵阳.聚乙烯亚胺改性聚丙烯腈中空膜固定脂肪酶[J].分子催化,2018,32(1):79-89 |
| 聚乙烯亚胺改性聚丙烯腈中空膜固定脂肪酶 |
| The Modification of Polyacrylonitrile Hollow Membrane by Polyethyleneimine to Immobilize Lipase |
| 投稿时间：2017-11-20 修订日期：2017-12-20 |
| DOI： |
| 中文关键词: 聚丙烯腈 表面改性 酶固定化 脂肪酶 |
| 英文关键词:polyacrylonitrile surface modification enzyme immobilization lipase |
| 基金项目:国家自然科学基金青年基金项目（No.31401674）（National Natural Science Foundation of China （No.31401674））；"十三五"国家重点研发计划项目（No.2016YFD0401400） |
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| 中文摘要: |
| 聚丙烯腈是富腈基的高分子聚合物，易修饰改性，广泛应用于膜分离应用.我们以聚丙烯腈中空膜为载体，采用化学法交联聚乙烯亚胺并固定脂肪酶，固定过程中引入海藻酸钠，用CaCl2进行后处理，得到固定化脂肪酶PAN-PEI-SA/E-CaCl2载酶量为31.70（mg enzyme）/（g support），酶活为50.20 U/（g support），15次重复使用可保留58.77%的酶活，与游离酶相比耐酸性和耐温性有所提高，相同条件下与Nov 435相比，酶活更高，这表明最终得到的固定化脂肪酶有良好的工业应用前景. |
| 英文摘要: |
| Polyacrylonitrile is a nitrile-rich polymer and easy to be surface modified, and it has been widely applied in membrane separation. In this study, polyacrylonitrile hollow membrane was used as a support of enzyme immobilization by chemically cross-linking polyethyleneimine. Sodium alginate was added during immobilization, and the immobilized lipase was post-treated by CaCl2. The amount of loading enzyme of PAN-PEI-SA/E-CaCl2 was 31.70 (mg enzyme)/(g support) and the activity as 50.20 U/(g support).After 15 cycles of reactions, the immobilized enzyme retained 58.77% of initial enzyme activity, and showed an improvement of heat and acid resistance compared to free lipase. When it is compared to commercialized Nov 435 under same condition, PAN-PEI-SA/E-CaCl2 has higher enzyme activity during 15 cycles of reactions. It shows the immobilized lipase with PAN after surface modification has industrial application prospects. |
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