| 林惠颖,辛嘉英,李春雨,孙立瑞,夏春谷.颗粒性甲烷单加氧酶分离纯化方法的研究进展[J].分子催化,2018,32(1):90-98 |
| 颗粒性甲烷单加氧酶分离纯化方法的研究进展 |
| Progress on Isolation and Purification of Particulate Methane Monooxygenase |
| 投稿时间：2017-11-07 修订日期：2017-12-20 |
| DOI： |
| 中文关键词: 颗粒性甲烷单加氧酶 分离纯化 酶活 甲烷氧化菌素 |
| 英文关键词:particulate methane monooxygenase isolation and purification activity methanobactin |
| 基金项目:国家自然科学基金（21573055）（NNSF of China （21573055））；哈尔滨商业大学创新团队项目（2016TD006） |
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| 中文摘要: |
| 颗粒性甲烷单加氧酶（pMMO）是甲烷氧化菌的特征酶之一，在生物催化方面具有广泛的应用前景，但由于其内膜蛋白的性质以及纯化过程中的不稳定性，使其生物化学性质、金属活性位点等方面仍存在许多未知和争议.着重总结了颗粒性甲烷单加氧酶的分离纯化方法，并对其活性以及与甲烷氧化菌素-Cu（methanobactin-Cu）和其他物质之间的作用关系进行了概述，以促进颗粒性甲烷单加氧酶的深入研究和应用. |
| 英文摘要: |
| Particulate methane monooxygenase (pMMO) is one of the main enzyme in methanotrophic bacteria, which has broad application prospect in biocatalysis. However, due to its characteristic of membrane protein and instability in purification procedures, there are many controversies in its biological and chemical properties and metal active sites. Purification methods of particulate methane monooxygenase were summarized. Besides, its activity and the relationship with methanobactin-Cu were introduced to improve the comprehensive research and application of particulate methane monooxygenase. |
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