| 李红霞,谢宗波,陈国庆,张灿,雷高乙,乐长高.酶促水解法拆分布洛芬乙酯的工艺研究[J].分子催化,2019,33(5):447-452 |
| 酶促水解法拆分布洛芬乙酯的工艺研究 |
| Study on the Process of Resolution of Ibuprofen Ethyl Ester by Enzymatic Hydrolysis |
| 投稿时间：2019-09-10 修订日期：2019-09-23 |
| DOI： |
| 中文关键词: S-布洛芬乙酯 曲拉通X-100 南极假丝酵母脂肪酶B 光学纯度 酶促拆分 |
| 英文关键词:S-ibuprofen ethyl ester triton X-100 lipase B from Candida antarctica optical purity enzymatic resolution |
| 基金项目:国家自然科学基金（21462001）；江西省创新团队专项（自然科学类）（20161BCB24006）；江西省教育厅科技项目（GJJ150584） |
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| 中文摘要: |
| 以布洛芬乙酯为反应底物，探索了酶促水解法拆分布洛芬的工艺.以曲拉通X-100为表面活性剂，利用南极假丝酵母脂肪酶B（CAL-B）催化拆分外消旋布洛芬乙酯，并对表面活性剂含量、有机溶剂种类和添加量、酶量、缓冲溶液pH、温度及时间等反应条件进行了优化，得到的最佳反应条件为：在pH为9.0的缓冲溶液中，添加80 mg曲拉通X-100、50 μL二氯甲烷和15 mg CAL-B，于30℃下反应48 h，在该条件下，S-布洛芬乙酯的剩余率（C）为65%，布洛芬乙酯的对映体过量值（ees）为94%. |
| 英文摘要: |
| The process of resolution of ibuprofen by enzymatic hydrolysis was explored using ibuprofen ethyl ester as the reaction substrate. The racemic ibuprofen ethyl ester was hydrolyzed by CAL-B (lipase B from Candida antarctica) using Triton X-100 as a surfactant. The reaction parameters such as surfactant content, organic solvent type, organic solvent content, enzyme loading, pH of buffer solution, temperature and time were optimized. The optimal reaction conditions were as follows:in a buffer solution of pH 9.0, 80 mg of Triton X-100, 50 μL of dichloromethane, and 15 mg of CAL-B were added and reacted at 30℃ for 48 h. Under the above conditions, the residual rate of S-ibuprofen ethyl ester (C) was 65% and the enantiomeric excess value of ibuprofen ethyl ester (ees) was 94%. |
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