| 尹一,孙立瑞,刘青云,辛嘉英.基于硼氢化钠原位还原的纳米金杂化酶的制备及催化阿魏酸甘油酯合成[J].分子催化,2024,38(1):26-34 |
| 基于硼氢化钠原位还原的纳米金杂化酶的制备及催化阿魏酸甘油酯合成 |
| Synthesis of Glycerol Ferulate Catalysed by NaBH4@AuNPs-CRL Hybridases Prepared by in situ Reduction of Sodium Borohydride |
| 投稿时间：2023-09-27 修订日期：2023-11-05 |
| DOI：10.16084/j.issn1001-3555.2024.01.004 |
| 中文关键词: 脂肪酶 纳米金 硼氢化钠 杂化酶 阿魏酸甘油酯 |
| 英文关键词:lipase gold nanoparticles sodium borohydride hybridases glyceryl ferulate |
| 基金项目:中央支持地方高校改革发展资金人才培养支持计划项目(No. 304017) |
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| 中文摘要: |
| 建立了一种基于硼氢化钠原位还原法制备纳米金杂化酶用以合成阿魏酸甘油酯的方法, 使杂化酶的催化活性、稳定性及结构的刚性皆有所提高. 利用硼氢化钠原位还原法制备了NaBH4@AuNPs-CRL杂化酶, 通过单因素实验得到了最优的杂化条件, 并利用荧光光谱、红外光谱、X-射线电子能谱、透射电镜等方法探究了杂化前后酶结构的变化. 研究表明制备的NaBH4@AuNPs-CRL杂化酶蛋白二级结构中α-螺旋含量减少、β-折叠含量增加, 杂化酶比活性为4.91 ±0.12 U·mg-1, 与游离酶相比提高了66.44%, 最佳条件下催化合成阿魏酸甘油酯的转化率为98.39% ±3.65%, 批式操作稳定性实验表明杂化酶的稳定性有较大提升. |
| 英文摘要: |
| In this work, a method based on the in situ reduction of sodium borohydride for the preparation of gold nanobiohybrids for the synthesis of glycerol ferulate was established, which resulted in better activity, stability and structural rigidity of the hybridases. The optimum conditions for the preparation of the NaBH4@AuNPs-CRL hybridases were obtained by a one-way experiment, and the changes of the lipase before and after hybridisation were explored using fluorescence spectroscopy, infrared spectroscopy, X-ray electron spectroscopy and transmission electron microscopy. It has been shown that the NaBH4@AuNPs-CRL hybridases have a reduction of α-helix and an increase of β-folding in their secondary structure. The specific activity of the NaBH4@AuNPs-CRL hybridases was 4.91 ±0.12 U·mg-1, which was 66.44% higher compared to CRL. The conversion of the glycerol ferulate synthesis catalysed by the NaBH4@AuNPs-CRL hybridases under the optimum conditions was 98.39% ±3.65%, and the stability experiments carried out in batch mode showed that both the activity and the stability had been improved. |
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